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Key Documents

MAB3262B

Sigma-Aldrich

Anti-BrdU Antibody, clone PRB-1, biotin conjugated

clone PRB-1, Chemicon®, from mouse

Synonyme(s) :

BrdU

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

biotin conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

PRB-1, monoclonal

Réactivité de l'espèce (prédite par homologie)

all

Fabricant/nom de marque

Chemicon®

Technique(s)

flow cytometry: suitable

Isotype

IgG1κ

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Spécificité

Recognizes BrdU in ssDNA, free BrdU or BrdU coupled to a protein carrier. MAB3262 also reacts with iodouridine. Does not react with thymidine.

Immunogène

Iodouridine conjugated to ovalbumin.

Application

Flow cytometry: 1:20

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-BrdU Antibody, clone PRB-1, biotin conjugated (Mouse Monoclonal Antibody) validated in FC to detect Bromodeoxyuridine also known as BrdU.

Forme physique

Purified immunoglobulin conjugated to biotin. Liquid in PBS, pH 7.4 containing 0.05% sodium azide.

Stockage et stabilité

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt. Protect from prolonged exposure to light.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Xingyu Lv et al.
Hepatology communications, 6(9), 2340-2353 (2022-05-06)
Pyroptosis is a kind of programmed cell death primarily mediated by gasdermin D (GSDMD) and shown to regulate multiple diseases. However, its contribution to liver regeneration, a fine-tuned tissue repair process mediated primarily by hepatocytes after mass loss, remains unclear.
R Sartaj et al.
Scientific reports, 7(1), 3793-3793 (2017-06-21)
In order to identify reliable markers of corneal epithelial stem cells, we employed an inducible transgenic "pulse-chase" murine model (K5Tta × TRE-H2BGFP) to localize, purify, and characterize slow cycling cells in the cornea. The retention of GFP labeling in slowly dividing cells
Katharine Cecchini et al.
Reproduction (Cambridge, England), 165(2), 183-196 (2022-11-18)
The testis-specific transcription factor, TCFL5, expressed in pachytene spermatocytes regulates the meiotic gene expression program in collaboration with the transcription factor A-MYB. In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the
Rebeca Piatniczka Iglesia et al.
Stem cell research & therapy, 8(1), 76-76 (2017-04-18)
Glioblastoma (GBM), a highly aggressive brain tumor, contains a subpopulation of glioblastoma stem-like cells (GSCs) that play roles in tumor maintenance, invasion, and therapeutic resistance. GSCs are therefore a promising target for GBM treatment. Our group identified the cellular prion

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