MAB1271-I

Anti-Golgi Zone Antibody, clone 371-4

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.74

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 371-4, monoclonal
• Poids mol.: calculated mol wt 134.55 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_001139138
• Numéro d'accès UniProt: Q92896
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GLG1(2734)

Description

Description générale

Golgi apparatus protein 1 (UniProt: Q92896; also known as CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160) is encoded by the GLG1 (also known as CFR1, ESL1, MG160) gene (Gene ID: 2734) in human. The Golgi apparatus plays a major role in transport, processing, and sorting of thousands of macromolecules in eukaryotic cells. It is readily recognizable because of its stacked membrane-coated cavities known as dictyosomes. The Golgi complex consists of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face and each of these regions contain various enzymes to selectively modify the macromolecules that pass through this complex. The Golgi complex is localized in the vicinity of the nucleus and is usually very large in secretory cells, where it fills most of the cytoplasm. Clone 371-4 selectively recognizes the Golgi complex in human cells. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells.

Spécificité

Clone 371-4 is a mouse monoclonal antibody that detects Golgi apparatus.

Immunogène

SU-DHL-1 human large cell lymphoma cells.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in HeLa cells.

Tested Application

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in A431cells.

Immunocytochemistry Analysis: A representative lot detected Golgi Zone in Immunocytochemistry applications (Endo, Y., et al. (2007). Cancer Sci.;98(10):1633-7; Strehlow, A.N., et al. (2007). Hum Mol Genet.;16(4):391-409; Oiso, S., et al. (2009). J Neurochem.;109(1):158-67; Leite, C., et al. (2014). PLoS One.;9(10):e111059).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Golgi Zone, clone 371-4, Cat. No. MAB1271-I, is a mouse monoclonal antibody that detects Golgi apparatus protein 1 and is tested for use in Immunocytochemistry.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable