ABE1953
Anti-PTRF/Cavin-1 Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
Polymerase I and transcript release factor, PTRF/Cavin-1, Cavin-1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat, human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CAVIN1(284119)
Description générale
Polymerase I and transcript release factor (UniProt Q6NZI2; also known as Cavin-1, PTRF, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12) is encoded by the PTRF (also known as CGL4, CAVIN, CAVIN1, FKSG13) gene (Gene ID 284119) in human. Cavin-1 is the founding member of the PTRF/Cavin family of cytoplasmic proteins whose expressions are essential for caveola formation. PTRF/Cavin-1, serum deprivation response (SDR)/Cavin-2, SDR-related gene product that binds to C kinase (SRBC)/Cavin-3, and muscle-restricted coiled-coil protein (MURC)/Cavin-4, form an oligomeric assembly termed Cavin complex in the cytosol and associate with caveolin at plasma membrane caveolae. Research shows an essential role for PTRF/Cavin-1 but not the other family members in caveola formation and recruitment of the cavin complex to the plasma membrane. In prostate cancer PC3 cells and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, where caveolin displays increased lateral mobility on the plasma membrane and accelerated lysosomal degradation. Expression of Cavin-1 restores caveolae formation in PC3 cells. Likewise the absence of cavin-1 is also observed in advanced prostate carcinoma. PTRF gene mutations are known to cause congenital generalized lipodystrophy 4 (CGL4).
Spécificité
Expected to react with spliced isoforms 1 and 2, but not isoform 3.
Immunogène
Linear peptide corresponding to the N-terminus of human PTRF/Cavin-1.
Application
Anti-PTRF/Cavin-1 Antibody is an antibody against PTRF/Cavin-1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry.
Western Blotting Analysis: A representative lot detected PTRF/Cavin-1 expression in HeLa, but not in prostate cancer cell lines, LNCaP, 22Rv1 and PC-3 (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Western Blotting Analysis: A representative lot detected exogenously expressed human PTRF/Cavin-1 in transfected 22Rv1 and LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunohistochemistry Analysis: A representative lot detected reduced PTRF/Cavin-1 immunoreactivity in paraffin-embedded malignant prostate stroma tissue samples when compared with normal prostate stroma and benign prostatic hyperplasia (BPH) specimens. A lack of PTRF/cavin-1 immunoreactivity in prostate epithelia was seen among both normal and malignant samples (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunocytochemistry Analysis: A representative lot detected surface caveola localization of exogenously expressed human PTRF/Cavin-1 among transfected LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Western Blotting Analysis: A representative lot detected exogenously expressed human PTRF/Cavin-1 in transfected 22Rv1 and LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunohistochemistry Analysis: A representative lot detected reduced PTRF/Cavin-1 immunoreactivity in paraffin-embedded malignant prostate stroma tissue samples when compared with normal prostate stroma and benign prostatic hyperplasia (BPH) specimens. A lack of PTRF/cavin-1 immunoreactivity in prostate epithelia was seen among both normal and malignant samples (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Immunocytochemistry Analysis: A representative lot detected surface caveola localization of exogenously expressed human PTRF/Cavin-1 among transfected LNCaP cells (Moon, H., et al. (2014). Oncogene 33(27):3561-3570).
Qualité
Evaluated by Western Blotting in rat skeletal muscle myoblast L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PTRF/Cavin-1 in 10 µg of rat skeletal muscle myoblast L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PTRF/Cavin-1 in 10 µg of rat skeletal muscle myoblast L6 cell lysate.
Description de la cible
~52 kDa observed.
Autres remarques
Concentration: Please refer to lot specific datasheet.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Bayader Annabi et al.
International journal of stem cells, 10(1), 103-113 (2016-12-28)
Tumour necrosis factor (TNF)-α activation of mesenchymal stromal cells (MSC) enhances their tumour-suppressive properties and tumour-homing ability. The molecular actors involved are unknown. We found that TNF induced MSC migration and tubulogenesis which correlated with a dose-dependent increase in Cavin-1
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