71077

X-Gal Solution - Novagen

Convenience for blue/white screening

• Synonyme(s) : X-Gal solution
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 100
• Forme: solution
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze
• Technique(s): tissue culture: suitable
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing.[1] X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.

Application

X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the Apc^Δ242 mutation in the tissue sample.[2]

Avertissement

Toxicity: Irritant (B)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 188.6 °F
• Point d'éclair (°C): 87 °C