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69451-M

Sigma-Aldrich

BL21(DE3)pLysS Competent Cells - Novagen

Escherichia coli, rod shaped

Synonyme(s) :

BL21 cell line, Novagen competent cells

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.81
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

BL21(DE3)pLysS Competent Cells - Novagen, BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

Source biologique

Escherichia coli

Niveau de qualité

100

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

transformation efficiency: >2×107 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

Composants

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBL21(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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BL21(DE3) Chemically Competent Cells for protein expression

Sigma-Aldrich

CMC0014

BL21(DE3) Chemically Competent Cells

E. coli BL21 Cytiva 27-1542-01, pack of 1 vial

GE27-1542-01

E. coli BL21

SIG10 5a Chemically Competent Cells for DNA plasmid production

Sigma-Aldrich

CMC0007

SIG10 5a Chemically Competent Cells

Debabrata Das et al.
Science advances, 6(44) (2020-11-01)
Oocyte numbers, a critical determinant of female reproductive fitness, are highly regulated, yet the mechanisms underlying this regulation remain largely undefined. In the Caenorhabditis elegans gonad, RAS/extracellular signal-regulated kinase (ERK) signaling regulates oocyte numbers; mechanisms are unknown. We show that

Questions

1–2 of 2 Questions  

  1. Does chloremphenicol need to be added to the culture media to maintain the presence of the pLys plasmid?

    1 answer

    1. The T7 lysozyme is expressed from a plasmid conferring resistance to chloramphenicol. 69451 will need to be cultured with chloramphenicol to maintain this plasmid.

      Helpful?

  2. What is the difference between the strain expressing pLysE and pLysS?

    1 answer

    1. Products 69451-M and 70236-M are exactly the same competent cells. The only difference is in the packaging. The 70236 is already in 50uL aliquots, which are single-use, each aliquot is used for one reaction. This reference is available in two sizes of 11 or 22 reactions. While product 69451 is available in single 0.4 or 1mL volumes which may be thawed once and dispensed to smaller aliquots.
      The difference between the 69451-M/70236-M products and the CMC0015 BL21 is the type of plasmid that they express for the T7 lysozymes. The expression of T7 lysozyme reduces the basal expression of the target genes, inhibiting the T7 RNA polymerase. Specifically, with the expression of the plasmid pLysE (Ref. CMC0015) a higher expression of the levels of T7 Lysozime is obtained in comparison with the plasmid pLysS (ref. 69451-M/70236-M). Consequently, with reference CMC0015 there is even less risk of basal expression of the target protein in the absence of IPTG stimulation. This could be particularly relevant for toxic proteins that could affect competent cell viability.

      Helpful?

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