69066-M

Recombinant Enterokinase

• Synonyme(s) : Enterokinase Enzyme, Enterokinase Protein, Recombinant Enterokinase
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Forme: liquid
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Technique(s): targeted protein degradation: suitable
• Adéquation: suitable for enzyme test
• Application(s): diagnostic assay manufacturing
• Température de stockage: −20°C

Description

Description générale

Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.

Composants

•50 URecombinant Enterokinase

•2 ml1X rEK Dilution/Storage Buffer

•1 ml10X rEK Cleavage Buffer

•10 µgCleavage Control Protein

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Définition de l'unité

One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.

Autres remarques

Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable