616387

N^α-Tosyl-Phe Chloromethyl Ketone

Irreversible inhibitor of chymotrypsin.

• Synonyme(s) : N^α-Tosyl-Phe Chloromethyl Ketone, TPCK
• Formule empirique (notation de Hill): C₁₇H₁₈ClNO₃S
• Numéro CAS: 402-71-1
• Poids moléculaire : 351.85
• Numéro MDL: MFCD00000935
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (TLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: white
• Solubilité: ethanol: 5 mg/mL; methanol: 5 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C17H18ClNO3S/c1-13-7-9-15(10-8-13)23(21,22)19-16(17(20)12-18)11-14-5-3-2-4-6-14/h2-10,16,19H,11-12H2,1H3/t16-/m0/s1
• Clé InChI: MQUQNUAYKLCRME-INIZCTEOSA-N

Description

Description générale

Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharide (EC₅₀ = 20 µM). Suggested working concentration is 10-100 µM.

This product has been discontinued.


Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides (EC₅₀ = 20 M).

Actions biochimiques/physiologiques

Cell permeable: no

EC50 = 20 µM in blocking the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides.

Primary Target
chymotrypsin activity in preparations

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 4 months at 4°C.

Autres remarques

Fearnhead, H.O., et al. 1995. FEBS Lett. 357, 242.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Weaver, V.M., et al. 1993. Biochem. Cell Biol.71, 488.
Murakami, T., et al. 1991. Biochim. Biophys. Acta1079, 279.
Nierodzik, M.L., et al. 1991. J. Clin. Invest.87, 229.
Wilson, W.E., et al. 1989. J. Biol. Chem. 264, 17777.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H318,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable