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Key Documents

5.08741

Millipore

DAPI Stain

Synonyme(s) :

DAPI Stain, 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O

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About This Item

Formule empirique (notation de Hill):
C16H15N5 · 2HCl
Numéro CAS:
Poids moléculaire :
350.25
Numéro MDL:
Code UNSPSC :
41116130
Nomenclature NACRES :
NA.21

Pureté

≥97% (HPLC)

Niveau de qualité

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

Fluorescence

λex ~359 nm
λem ~461 nm

Température de stockage

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Clé InChI

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description générale

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cells are typically stained at 1 µg/ml.

Application


  • Determination of Ploidy Levels and Nuclear DNA Content in Cryptococcus neoformans by Flow Cytometry: Drawbacks with Variability: This research utilizes DAPI for quantifying nuclear DNA content and determining ploidy levels in Cryptococcus neoformans, providing critical insights into fungal genetics and potential challenges in reproducibility (Chang et al., 2024).

  • Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe: Highlights the development of a novel two-photon fluorescence probe for nucleic acid imaging, employing DAPI as a reference for comparing fluorescence characteristics, enhancing molecular imaging techniques (Sun et al., 2024).

  • A FACS-based Novel Isolation Technique Identifies Heterogeneous CTCs in Oral Squamous Cell Carcinoma: Applies DAPI for the enumeration and characterization of circulating tumor cells (CTCs), showcasing its importance in cancer diagnostics and the potential for personalized medicine approaches (Chauhan et al., 2024).

Actions biochimiques/physiologiques

Cell permeable: yes
Primary Target
DNA
Reversible: yes

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Forme physique

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Autres remarques

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.

Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.

Russels, W.C., et al. 1975. Nature253, 461.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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