219364

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• Synonyme(s) : Cathepsin B, Human Liver
• Numéro CAS: 9047-22-7
• Numéro de classification (Commission des enzymes): 3.4.22.1 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human liver
• Niveau de qualité: 100
• Pureté: >95% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥50 units/mg protein
• Produit purifié par: affinity chromatography
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Technique(s): activity assay: suitable
• Plage de pH: 3.5—6.0
• Adéquation: suitable for molecular biology
• Application(s): life science and biopharma
• Conditions d'expédition: wet ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CTSB(1508)

Description

Description générale

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.

Optimal pH: 3.5-6.0.

Application

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.

Actions biochimiques/physiologiques

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

Forme physique

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Notes préparatoires

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable