14-440-M

PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli; expressed in BL21 DE3 cells
• Gamme de produits: Upstate^®
• Forme: liquid
• Activité spécifique: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.); 8580 U/mg
• Durée de conservation: 1 yr
• Poids mol.: M_w 40.7 kDa
• Produit purifié par: chromatography
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable (kinase)
• Solubilité: water: soluble
• Numéro d'accès: (FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. ); NM_002730.3
• Numéro d'accès NCBI: NM_002730.3; NM_207518.1
• Numéro d'accès UniProt: P17612
• Température de stockage: −70°C
• Informations sur le gène: human ... PRKACA(46909581)

Description

Description générale

Research area: Cell Signaling

Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.

Recombinant human full length PKA, catalytic subunit type alpha, untagged

Actions biochimiques/physiologiques

Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.

Conditionnement

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Qualité

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Description de la cible

PKA

Liaison

Replaces: 14-114

Forme physique

Phosphocellulose P11 chromatography

Stockage et stabilité

1 year at -70°C

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1