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1.09215

Sigma-Aldrich

Ziehl-Neelsen carbol-fuchsin solution

for microscopy

Synonyme(s) :

Carbol fuchsin solution

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About This Item

Code UNSPSC :
41116130
Nomenclature NACRES :
NA.74

Niveau de qualité

400

Forme

liquid

IVD

for in vitro diagnostic use

Température de transition

flash point 47 °C

Densité

0.99 g/cm3 at 20 °C

Application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

Température de stockage

15-25°C

Description générale

Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer′s methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Product number 1.00497).
The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For Microscopy. Ready-to-use staining solution for the purpose of bacteriological and histological investigation of sample material of human origin.

Informations légales

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Mention d'avertissement

Danger

Mentions de danger

H226 - H314 - H341 - H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

116.6 °F

Point d'éclair (°C)

47 °C

Certificats d'analyse (COA)

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