09-271
Anti-Rac1b Antibody
Upstate®, from rabbit
Synonyme(s) :
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human
Fabricant/nom de marque
Upstate®
Technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... RAC1(5879)
Description générale
Ras-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Spécificité
This rabbit polyclonal antibody specifically detects Rac1b in human and murine cells.
Immunogène
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Rac1b.
Application
Anti-Rac1b Antibody, Cat. No. 09-271, is a rabbit polyclonal antibody that detects Ras-related C3 botulinum toxin substrate 1 (Rac1b) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Rac1b in HeLa, A431, HUVEC, and NIH/3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.
Qualité
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.
Description de la cible
~17 kDa observed; 23.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Hendrik Ungefroren et al.
Cancers, 11(5) (2019-05-22)
The small GTPase Ras-related C3 botulinum toxin substrate 1B (RAC1B) has been shown previously by RNA interference-mediated knockdown (KD) to function as a powerful inhibitor of transforming growth factor (TGF)-β1-induced cell migration and epithelial-mesenchymal transition in epithelial cells, but the
Huizi Wu et al.
International journal of cancer, 143(11), 2962-2972 (2018-08-16)
Recent studies suggest that malignant melanoma heterogeneity includes subpopulations of cells with features of multipotent neural crest (NC) cells. Zebrafish and mouse models have shown that reactivation of neural crest-specific pathways during transformation determines the invasiveness of melanoma cells. In
Ibuprofen inhibits colitis-induced overexpression of tumor-related Rac1b.
Matos, P; Kotelevets, L; Goncalves, V; Henriques, AF; Henriques, A; Zerbib, P; Moyer et al.
Neoplasia null
Christine Mehner et al.
Genes & cancer, 6(11-12), 480-489 (2016-01-26)
Breast, lung, and pancreatic cancers collectively represent one third of all diagnosed tumors and are responsible for almost 40% of overall cancer mortality. Despite improvements in current treatments, efforts to develop more specific therapeutic options are warranted. Here we identify
Matrix metalloproteinase induction of Rac1b, a key effector of lung cancer progression.
Stallings-Mann, ML; Waldmann, J; Zhang, Y; Miller, E; Gauthier, ML; Visscher, DW; Downey et al.
Science Translational Medicine null
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