05-414
Anti-CrkL Antibody, clone 5-6
clone 5-6, Upstate®, from mouse
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
5-6, monoclonal
Espèces réactives
mouse, rat, human
Fabricant/nom de marque
Upstate®
Technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CRKL(1399)
Description générale
CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.
The antibody does not cross-react with CRKI or CRKII.
Spécificité
Other species have not been tested.
the C-terminal SH3 of Human CRKL
Immunogène
Epitope: Full-length
Full length GST fusion protein corresponding to Human CRKL
Application
Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Qualité
Routinely evaluated by Western Blot on K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Description de la cible
39kDa
Forme physique
Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Remarque sur l'analyse
Control
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway
Sattler, M, et al
Oncogene, 12, 839-846 (1996)
Atsushi Oda et al.
The Journal of biological chemistry, 278(8), 6456-6460 (2003-01-11)
Searching for proteins in platelets that can interact with the N-terminal SH3 domain of CrkL (using a combination of a pull-down assay followed by mass spectrometry), we have found that human platelets express an ADP-ribosylation factor (Arf)-specific GTPase-activating protein (GAP)
IL-4 regulates the expression of CD209 and subsequent uptake of Mycobacterium leprae by Schwann cells in human leprosy.
Teles RM, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, Modlin RL
Infection and Immunity null
Eike R Hrincius et al.
Cellular microbiology, 12(6), 831-843 (2010-01-22)
The non-structural protein 1 (A/NS1) of influenza A viruses (IAV) harbours several src-homology domain (SH) binding motifs that are required for interaction with cellular proteins. The SH3 binding motif at aa212-217 [PPLPPK] of A/NS1 was shown to be essential for
Cellular interactions of CRKL, and SH2-SH3 adaptor protein
ten Hoeve, J, et al
Cancer Research, 54, 2563-2567 (1994)
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