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Key Documents

04-119-S

Sigma-Aldrich

Anti-acetyl-Histone H4 (Lys12) Antibody, Trial Size, rabbit monoclonal

culture supernatant, Chemicon®

Synonyme(s) :

H4K12Ac, Histone H4 (acetyl K12)

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

culture supernatant

Type de produit anticorps

primary antibodies

Clone

monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ChIP: suitable
dot blot: suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

NM_003538.3

Numéro d'accès UniProt

P62805

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

acetylation (Lys12)

Informations sur le gène

human ... H4C1(8359)

Spécificité

Recognizes Histone H4 when acetylated on Lys12.
Wide range of cross-reactivity expected based on sequence homology.

Immunogène

Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.

Application

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
This Anti-acetyl-Histone H4 (Lys12) Antibody, Trial Size / Pack is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).

Qualité

Routinely evaluated by immunoblot.

Description de la cible

11kDa

Forme physique

10 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide.

Stockage et stabilité

2 years at -20°C from date of shipment

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Michael S Werner et al.
Nature communications, 14(1), 2095-2095 (2023-04-14)
Development can be altered to match phenotypes with the environment, and the genetic mechanisms that direct such alternative phenotypes are beginning to be elucidated. Yet, the rules that govern environmental sensitivity vs. invariant development, and potential epigenetic memory, remain unknown.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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