Accéder au contenu
Merck
Toutes les photos(2)

Documents

WHA10401316

Whatman® Protran® nitrocellulose membranes

Circles - BA83, pore size 0.2 μm, diam. 82 mm

Synonyme(s) :

Z670618, blotting membrane, nitrocellulose membrane, transfer membrane

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41105339
Nomenclature NACRES :
NB.22

Conditionnement

pkg of 50 ea

Fabricant/nom de marque

Whatman 10401316
Whatman Article No. 28415007 (US reference)

Diamètre

82 mm

Dimension de pores

0.2 μm pore size

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Manufactured using 100% pure nitrocellulose to ensure the highest binding capacity. The surface properties of the membrane ensure superior signal-to-noise ratios without the need for stringent washing conditions. The proprietary Protran nitrocellulose formula is proven to provide excellent shelf life of proteins. Evidence shows proteins maintain molecular recognition activity for five years on Protran.

  • Binding capacity, 75 - 110 μg/cm2
  • High binding, low background
  • Triton free matrix
  • Universal membrane for blotting - isotopic, chemluminescent, colorimetric and fluorescent detection methods

Protran nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as ‘nitrocellulose′ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes.

They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric and fluorescent. Unlike PVDF membranes, Protran nitrocellulose does not require a methanol pre-wetting step. This makes Protran the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pre-treatment steps are necessary.

High Binding, Low Background:
In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.

High Retention of Small Proteins:

The 0.2 μm pore size of the Protran (BA83) nitrocellulose membrane has high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through′. The 0.45 μm pore size membrane (BA85) is ideal for larger molecular weight samples. BA79, with 0.1 μm pore size, is the membrane of choice for smaller proteins below 7 kD. A unique benefit of the proprietary Protran nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran.

Protran Blotting Sandwiches:

A pre-cut nitrocellulose membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time. This is made with our Protran BA83 or BA85, the highest quality NC membranes available for blotting.

Autres remarques

Field of Use : Any microbiological or biotechnological application, including, but not limited to application of membranes for diagnostic, medical, biological, pharmaceutical, consumer product and food and beverage purposes.

Informations légales

Nitrocellulose Membranes (FFHP, Immunopore and BA membranes)
Sold under license to DE10102744 and foreign equivalents thereof
Protran is a registered trademark of Cytiva
Whatman is a registered trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Conseils de prudence

Classification des risques

Flam. Sol. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique