TRUE6
TrueGel3D Hydrogel Kits
SLO-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker
Synonym(s):
3D Hydrogel Kit, TrueGel3D Kit
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About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice
shipped in
dry ice
storage temp.
−70°C
General description
The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-DEXTRAN does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
Application
TRUE6-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic natural tissue stiffness and to investigate the effect of mechanical stimulus on cell physiology and behavior. It is also used to culture non-proliferating cells and proliferating spheroids that do not attach to hydrogel for survival, but rather use their own extracellular matrix.
Features and Benefits
- Slow gelling (takes 8 minutes to 25 minutes, depends on gel stiffness)
- Flexibility in modulating gel stiffness
- Does not allow cell migration
- Allow cell recovery and post-culture analyses
- Cell inert hydrogel (if not customized with RGD peptide)
Other Notes
TRUE6-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.
Disclaimer
Avoid prolong exposure of PEG non cell-degradable crosslinker to air.
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
wgk
WGK 3
Storage Class
10 - Combustible liquids
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