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SRP3142

Sigma-Aldrich

PF-4 (CXCL4) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

CXCL4, Ironplact, Oncostatin A, Platelet Factor-4

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-10.0 ng/mL

mol wt

7.8 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P02776

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PF4(5196)

Related Categories

General description

Platelet factor-4 (PF-4) is a CXC chemokine that is expressed in megakaryocytes and stored in the alpha-granules of platelets.[1] The gene encoding this protein is localized on human chromosome 4.[2] Recombinant human PF-4 is a 7.8kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines.

Biochem/physiol Actions

Platelet factor-4 (PF-4) is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis.[3] This protein associates with heparin and neutralizes it. It also neutralizes various negatively charged proteoglycans.[4]

Sequence

EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
101543
060843
07043

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Chemokines and Chemokine Receptors
Manel Juan
Wiley Guide to Chemical Incompatibilities (2013)
B Scheuerer et al.
Blood, 95(4), 1158-1166 (2000-02-09)
Unstimulated monocytes rapidly undergo physiological changes resulting in programmed cell death (apoptosis) while stimuli promoting differentiation of these cells into macrophages were shown to inhibit apoptotic processes. In the present study, we report that the platelet-derived alpha-chemokine platelet factor 4
CXCL4 Plasma Levels Are Not Associated with the Extent of Coronary Artery Disease or with Coronary Plaque Morphology.
Erbel C
PLoS ONE, 10(11), e0141693-e0141693 (2015)
S S Huang et al.
The Journal of biological chemistry, 257(19), 11546-11550 (1982-10-10)
A large scale purification procedure for the human platelet factor 4 proteoglycan carrier molecule has been developed. A yield of 46% and a 33,000-fold purification have been achieved, using poly-L-lysine-Sepharose affinity column chromatography, PF4-agarose affinity column chromatography, and Bio-Gel A-0.5m
Yong Du et al.
The Journal of experimental medicine, 219(7) (2022-06-01)
Chemokines control the migratory patterns and positioning of immune cells to organize immune responses to pathogens. However, many chemokines have been associated with systemic autoimmune diseases that have chronic IFN signatures. We report that a series of chemokines, including CXCL4
View All Related Papers

Questions

  1. can this protein work on mouse?

    1 answer

    1. This product is tested for activity using human fibroblasts. A brief search of the literature could not confirm that the human source protein would be fully bioactive in mice. However, product SRP3231 is PF-4 from mouse and is tested on human neutrophils. While it is likely that mouse cells would respond to the human chemokine, it would be up to the end user to confirm suitability. See the links below to review a sample Certificate of Analysis for each product.

      SRP3142 Human PL-4
      https://www.sigmaaldrich.com/coa/SIGMA/SRP3142/101543

      SRP3231 Mouse PF-4
      https://www.sigmaaldrich.com/coa/SIGMA/SRP3231/1208471

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