This antibody has been tested for use in flow cytometry at a concentration of 2.5-5 µg /test using SK-Mel-28 cells and immunofluorescence at a concentration of 2.5-5 µg/mL using A375 cells. It has not been validated for use in ELISA. The cross-reactivity has not been investigated. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/255/822/sab4200621dat.pdf
SAB4200621
Anti-CSPG4 Antibody
mouse monoclonal, 2164B6
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.
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Product Name
Monoclonal Anti-NG2/CSPG4 antibody produced in mouse, clone 2164B6, purified from hybridoma cell culture
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2164B6, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
flow cytometry: 2.5-5 μg/test using SK-Mel-28 cells.
immunofluorescence: 2.5-5 μg/mL using A375 cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CSPG4(1464)
General description
Nerve/glial antigen 2 (NG2), a high molecular mass melanoma- associated antigen (HMW-MAA), is a large integral membrane, cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan.
Immunogen
a recombinant extracellular portion of human NG2 proteoglycan
Application
Monoclonal Anti-NG2/CSPG4 antibody produced in mouse used in flow cytometry and immunocytochemistry.
Biochem/physiol Actions
Nerve/glial antigen 2 (NG2) plays a central role in linking multiple oncogenic pathways required for malignant progression. NG2 expression in radial growth phase (RGP) human melanomas enables migration, protease activation and epithelial to mesenchymal transition, which is important in primary tumors for facilitating progression from a radial to vertical growth phase phenotype. It regulates sustained, high-level activation of key survival and growth pathways, integrin-regulated focal adhesion kinase (FAK), extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and phosphoinositide 3-kinase (PI3K)/AKT pathways.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Megan S Lord et al.
The FEBS journal, 280(10), 2490-2510 (2013-02-20)
Proteoglycans are ubiquitous dynamic molecules that are made up of a protein core to which specific linear glycosylation structures, known as glycosaminoglycans, have been covalently coupled. They have roles in many biological and pathological processes, which have been shown to
Matthew A Price et al.
Pigment cell & melanoma research, 24(6), 1148-1157 (2011-10-19)
Chondroitin sulfate proteoglycan 4 (CSPG4), a transmembrane proteoglycan originally identified as a highly immunogenic tumor antigen on the surface of melanoma cells, is associated with melanoma tumor formation and poor prognosis in certain melanomas and several other tumor types. The
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there is no information provided about assay specificity and possible cross-reactions. please give me information about this. another question could we use ELİSA with this antibody?
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