Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.
Application
Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.
Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 12(13), 1359-1370 (1998-10-08)
Selenium deficiency is known to be associated with male infertility, and the selenoprotein PHGPx has been shown to increase in rat testis after puberty and to depend on gonadotropin stimulation in hypophysectomized rats [Roveri et al. (1992) J. Biol. Chem.
Journal of chromatography. A, 679(1), 67-83 (1994-09-09)
Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), produced as inclusion bodies in genetically transformed Escherichia coli cells was purified to homogeneity by a three-step chromatographic procedure involving hydrophobic interaction, ion exchange and gel filtration. Each purification step is reproducible and well
Journal of biotechnology, 113(1-3), 137-149 (2004-09-24)
A new technology for renaturation with simultaneous purification of the recombinant human interferon-gamma (rhIFN-gamma) in downstream of biotechnology is presented. The strategies to develop the new technology in industry scale were suggested. Based on chemical equilibrium and molecular interactions, the
Journal of chromatography. B, Biomedical sciences and applications, 737(1-2), 143-150 (2000-02-19)
A kinesin gene has been cloned by RT-PCR (reverse transcription polymerase chain reaction) from Trypanosoma brucei and the corresponding protein overexpressed as a recombinant His-tag (histidine-tag) kinesin in E. coli in order to study its biochemical properties and to determine
The major laccase isoenzyme LAP2 secreted by the white-rot basidiomycete Trametes pubescens in response to high copper concentrations was purified to apparent electrophoretic homogeneity using anion-exchange chromatography and gel filtration. The monomeric protein has a molecular mass of 65 kDa
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.