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Key Documents

RAB0490

Sigma-Aldrich

Human soluble TNF Receptor 2 ELISA Kit

for serum, plasma for serum, plasma, cell culture supernatant and urine

Synonym(s):

sTNFRII

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

The Human sTNF RII ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human sTNF RII in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TNF RII

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Tumor necrosis factor receptor 2 (TNFR2) plays a vital role in inducing signals for various cellular process, including proliferation of thymocytes and peripheral T cells, T cell secretion of granulocyte-macrophage colony-stimulating factor, inhibition of early hematopoiesis, and the inactivation and clearance of TNF by the kidney. Mutation in the gene leads to hematopoietic cell malignancies, neuroblastoma, glioma, cervical and ovarian carcinoma. TRAF2 interacts with TNFR2 and activates NF-kappa B (NF-κB) pathway. TNFR2, which lacks death domains, modulates the effect of other death receptor TNFR1, which induces T cell death. TNFR2 plays an essential role in TNF-α–induced leukocyte-endothelial interaction. TNFR2 is considered as a potential therapeutic alternative, as it has capacity to nullify the common side effects associated with drugs targeting TNF-α or p55.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tumor necrosis factor alpha (TNF-alpha) receptor-II is required for TNF-alpha-induced leukocyte-endothelial interaction in vivo.
Chandrasekharan UM, et al.
Blood, 109(5), 1938-1944 (2007)
The TNF and TNF receptor superfamilies: integrating mammalian biology.
Locksley RM, et al.
Cell, 104(4), 487-501 (2001)
Human tumor necrosis factor receptor p75/80 (CD120b) gene structure and promoter characterization.
Santee SM, et al.
The Journal of Biological Chemistry, 271(35), 21151-21159 (1996)
TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40.
Rothe M, et al.
Science, 269(5229), 1424-1427 (1995)

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