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F4503

Sigma-Aldrich

Ferritin from equine spleen

Type I, saline solution

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About This Item

CAS Number:
9007-73-2
MDL number:
MFCD00081593
UNSPSC Code:
12352202
NACRES:
NA.77
Pricing and availability is not currently available.

sterility

sterile-filtered

Quality Level

300

type

Type I

form

saline solution

mol wt

H subunit ~21 kDa
L subunit ~19 kDa

color

red to brown

cation traces

Cd: ≤1% (as % of ferritin)

storage temp.

2-8°C

Related Categories

General description

A shell of 24 protein subunits (apoferritin) and a core Fe 3+ ions with a peptide MW of 440 kDa. Review of the ferritin-like superfamily of iron-storage proteins, virtually ubiquitous in life on earth.
A shell of 24 protein subunits (apoferritin) and a core of Fe3+ ions with a peptide MW of 440 kDa.

Application

Ferritin from equine spleen has been used:
  • in mycobacterial growth assay[1]
  • in size-exclusion chromatography[2][3]
  • in labeling of macrophage cells[4]
  • as a reference standard in muon spin spectroscopy[5]

Biochem/physiol Actions

A ubiquitous iron storage protein that plays a key role in iron metabolism. It serves as an intracellular iron reserve (particularly in spleen, liver, intestinal mucosa, and bone marrow) and functions in iron detoxification.
A ubiquitous iron storage protein that plays a key role in iron metabolism. It serves as an intracellular iron reserve (particularly in spleen, liver, intestinal mucosa, and bone marrow) and functions in iron detoxification. Studies have shown that ferritin iron incorporation is mediated by a ferroxidase activity associated with ferritin H subunits and a nucleation center associated with ferritin L subunits. Release of iron from ferritin has an essential role in iron-dependent lipid peroxidation and may contribute to free radical-induced cell damage in vivo. Therefore, by binding iron, ferritin may function as an antioxidant.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000393695
0000387686
0000387687
0000355351
0000365446

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Human-brain ferritin studied by muon spin rotation: a pilot study
Bossoni L, et al.
Journal of Physics. Condensed Matter : An Institute of Physics Journal, 29(41), 415801-415801 (2017)
The effectiveness of ferritin as a contrast agent for cell tracking MRI in mouse cancer models
Lee CW, et al.
Yonsei Medical Journal, 58(1), 51-58 (2017)
Italo Moglia et al.
Journal of inorganic biochemistry, 183, 184-190 (2017-12-28)
Ferritin is a globular protein that consists of 24 subunits forming a hollow nanocage structure that naturally stores iron oxyhydroxides. Elimination of iron atoms to obtain the empty protein called apoferritin is the first step to use this organic shell
Defining the Architecture of the Core Machinery for the Assembly of Fe-S Clusters in Human Mitochondria
Methods in Enzymology, 595, 107-160 (2017)
Yves Gossuin et al.
Magnetic resonance in medicine, 48(6), 959-964 (2002-12-05)
Ferritin, the iron-storing protein, speeds up proton transverse magnetic relaxation in aqueous solutions. This T(2) shortening is used in MRI to quantify iron in the brain and liver. Current theoretical models underestimate the relaxation enhancement by ferritin at imaging fields
View All Related Papers

Questions

1–8 of 8 Questions  

  1. Hi, I've just purchased a Ferritin from equine spleen (liquid, 100mg, cas No:9007-73-2, MDL No: MFCD00081593), however, I didn't find how many mL this solution is on the product label. Can you please provide any information regarding volume? Thanks.

    1 answer

    1. The concentration, and thus the volume, of the Ferritin will vary for individual lots. The concentration can be found on the lot specific Certificate of Analysis, which can be retrieved by entering the lot number here:
      https://www.sigmaaldrich.com/product/sigma/f4503#product-documentation

      The volume can be determined using the following formula:
      Units provided / Units per mL = mL provided

      The concentration of the Ferritin is currently specified to be ≤125mg/ml
      https://www.sigmaaldrich.com/specification-sheets/115/477/F4503-BULK________SIGMA____.pdf

      Helpful?

  2. Does it run as a 44kDa specie or 21kDa+19kDa in size exclusion chromatography?

    1 answer

    1. This product is not analyzed by chromatography. However, historical data indicates the material runs primarily at 20-22kD and 13-15kD in SDS electrophoresis studies. Additional bands appear at the 3000 - 5000 molecular weight range.

      Helpful?

  3. What is the concentration of salt(s) in the saline solution carrying the protein?

    1 answer

    1. The saline solution is a 0.15 M sodium chloride solution. The concentration of 0.15 M can also be written as 8.8 mg NaCl per mL, or the textbook "0.9%" for normal saline.

      Helpful?

  4. Hi, I was wondering if I could get the volume of ferritin present in each of the package sizes? Thank you

    1 answer

    1. The concentration of this product is lot specific and will impact the final volume received. Typical lots range from 50 - 75 mg/mL. Please see the link below to review the product specification sheet:
      https://www.sigmaaldrich.com/specification-sheets/115/477/F4503-BULK________SIGMA____.pdf

      See below for potential volume ranges for each package size:
      F4503-25MG (0.5 - 0.33 mL)
      F4503-100MG (2 - 1.33 mL)
      F4503-500MG (10 - 6.66 mL)
      F4503-1G (20 - 13.33 mL)

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. Has Product F4503, Ferritin from equine spleen, been cell culture tested?

    1 answer

    1. The solution is sterile-filtered but has not been tested in cell culture.

      Helpful?

  7. Where is iron located in the ferritin molecule of Product F4503, Ferritin from equine spleen?

    1 answer

    1. Iron is located in the core of the ferritin molecule consisting of 24 subunits. Up to 4500 iron ions can be contained in this core.

      Helpful?

  8. What is the concentration of ferritin in solutions of Product F4503, Ferritin from equine spleen?

    1 answer

    1. The concentration of ferritin is lot specific and can be found on the Certificate of Analysis (CofA) for the specific lot.

      Helpful?

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