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CRISPRICON

Sigma-Aldrich

Human CRISPRi Control and Effector Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NE.02

packaging

pkg of 12 vials (4x50μL aliquots for each of the 3 kit components)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

General description

The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.

Application

  • Set up and optimization of screen assay
  • Functional Genomics/Target Validation
  • Creation of cell lines stably expressing KRAB-dCas9
  • Validated Positive and negative controls

Features and Benefits

  • Ease of optimization: Validated positive control targeting RAB1A and non-targeting control for assay set up
  • Best in Class UCSF gRNA selection algorithm and optimized (F+E) gRNA scaffold
  • UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines
  • Libraries provided at high functional titer, based on FACs or CFU analysis
  • Use puromycin and or BFP as selection markers

Components

2 Controls and 1 Effector Construct with a minimum concentration of 5x105 TU/mL(via FACS or CFU assay)

CRISPRIE: KRAB-dCas9 - Blasticidin CRISPRi Construct Lentiviral Transduction Particles
CRISPRI01: CRISPRi RAB1A Puromycin Control Transduction Particles
CRISPRI06: CRISPRi Nontargeting Control Puromycin Transduction Particles

For more information about screening with CRISPRi/a please Click Here.

Principle

The power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole genome, loss-of-function (LOF) screening, has allowed for new breakthroughs identifying gene pathways in drug resistance and disease. CRISPR is most commonly used to create double-stranded breaks that often result in loss of gene function (CRISPR-KO). However, the full extent of CRISPR′s utility extends beyond just targeted cutting of DNA. Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi. We partnered with University of California San Francisco to provide the best-in-class CRISPRi screening tools available.

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

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Articles

CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.

CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.

CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.

CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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