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43925

Sigma-Aldrich

Nigrosin Stain solution

for microscopy

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

Bacillus spp.
Corynebacterium spp.

General description

Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.

Components

Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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S C Esteves et al.
Fertility and sterility, 66(5), 798-804 (1996-11-01)
To determine whether the hypo-osmotic swelling test can assess viability of frozen spermatozoa. Fresh semen specimens were divided into two aliquots: one was not processed and the other was processed by swim-up. Both aliquots were then cryopreserved by the liquid
Emmanuel I Odongo-Aginya et al.
African health sciences, 7(1), 33-36 (2007-07-03)
The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the
R R Valle et al.
Theriogenology, 70(1), 115-120 (2008-05-16)
Simple, rapid and stable sperm evaluation methods which have been optimized for common marmoset (Callithrix jacchus) are critical for studies involving collection and evaluation of sperm in the field. This is particularly important for new species groups such as Callitrichidae
A L Fedorovtsev
Sudebno-meditsinskaia ekspertiza, 35(2), 28-30 (1992-04-01)
The morphology of isolated cells of multi-layer squamous non-horny epithelium (buccal, vaginal, urethral, rectal), stained with 0.05% nigrosine solution, was under study, and the sizes of these cells and their nuclei were measured. Specific markers were detected in the buccal
Jesse Schallek et al.
Investigative ophthalmology & visual science, 52(3), 1325-1335 (2010-11-06)
To examine the extent to which neurovascular coupling contributes to stimulus-evoked intrinsic signals in the retina. The retinas of five adult cats were examined in vivo. Animals were anesthetized and paralyzed for imaging stability. The retinas were imaged through a

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