p46Shc, p52Shc, p66Shc, SHC-transforming protein 1, SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1
SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.
Specificity
Clone 3A12.1 was raised against a C-terminal region sequence present in all six human SHC1 spliced isoforms reported by UniProt (P29353).
Immunogen
GST-tagged recombinant human Shc C-terminal region fragment.
Application
Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Shc proteins in human prostate cancer, as well as in normal breast and tonsil tissue sections.
Quality
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Shc proteins in 10 µg of SH-SY5Y cell lysate.
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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