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MAB030

Sigma-Aldrich

Anti-DNA Antibody, double stranded, clone BV16-13

culture supernatant, clone BV16-13, Chemicon®

Synonym(s):

Double-stranded DNA antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

BV16-13, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable

isotype

IgG2aκ

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

BINDING ASSAY:

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



ANTIGEN BINDING

PROFILE: Relative Binding To:



Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

Immunogen

Epitope: double stranded, 28% reactive with single stranded

Application

Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
EIA/RIA

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Apoptosis - Additional

Physical form

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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