AB755P
Anti-Rat Collagen Type I Antibody
Chemicon®, from rabbit
Synonym(s):
Anti-CAFYD, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
isotype
IgG
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... COL1A1(1277)
Related Categories
General description
Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.
Specificity
Recognizes Rat Collagen Type I
The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.
Immunogen
Collagen Type I extracted and purified from rat skin.
Application
Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.
ELISA:
A 1:200 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in RIA.
Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.
Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.
Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.
A 1:200 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in RIA.
Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.
Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.
Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.
Quality
Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.
IHC-Paraffin Staining With Epitope Retrieval: Rat Skin
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.
IHC-Paraffin Staining With Epitope Retrieval: Rat Skin
Physical form
The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.
Analysis Note
Control
Rat hepatic stellate cell lysate
Rat hepatic stellate cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Alan S Liu et al.
Scientific reports, 6, 33919-33919 (2016-09-28)
The biomechanical behavior of tissues under mechanical stimulation is critically important to physiological function. We report a combined experimental and modeling study of bioengineered 3D smooth muscle microtissues that reveals a previously unappreciated interaction between active cell mechanics and the
Chen-wen Li et al.
International journal of nanomedicine, 11, 373-386 (2016-02-09)
Wound healing occupies a remarkable place in everyday pathology and remains a challenging clinical problem. In our previous study, we prepared a silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) (PVA/COS-AgNPs) nanofiber via electrospinning and revealed that it could promote wound healing; however, the
Cathryn D Peltz et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 29(6), 874-879 (2011-01-20)
Damage to the biceps tendon is often seen in conjunction with rotator cuff tears. However, controversy exists regarding its role in the shoulder and its optimal treatment. A previous study determined that biceps tendons were detrimentally affected in the presence
An Autologous Muscle Tissue Expansion Approach for the Treatment of Volumetric Muscle Loss.
Ward, CL; Ji, L; Corona, BT
BioResearch Open Access null
Calcospherulites isolated from the mineralization front of bone induce the mineralization of type I collagen.
Midura, RJ; Vasanji, A; Su, X; Wang, A; Midura, SB; Gorski, JP
Bone null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service