- Inhibition of family II pyrophosphatases by analogs of pyrophosphate and phosphate.
Inhibition of family II pyrophosphatases by analogs of pyrophosphate and phosphate.
Biochemistry. Biokhimiia (2005-10-11)
A B Zyryanov, R Lahti, A A Baykov
PMID16212547
RESUMEN
Imidodiphosphate (the pyrophosphate analog containing a nitrogen atom in the bridge position instead of oxygen) is a potent inhibitor of family II pyrophosphatases from Streptococcus mutans and Streptococcus gordonii (inhibition constant Ki approximately 10 microM), which is slowly hydrolyzed by these enzymes with a catalytic constant of approximately 1 min(-1). Diphosphonates with different substituents at the bridge carbon atom are much less effective (Ki = 1-6 mM). The value of Ki for sulfate (a phosphate analog) is only 12 mM. The inhibitory effect of the pyrophosphate analogs exhibits only a weak dependence on the nature of the metal ion (Mn, Mg, or Co) bound in the active site.
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