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Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.

Science advances (2020-04-10)
Stephen D Carter, Cheri M Hampton, Robert Langlois, Roberto Melero, Zachary J Farino, Michael J Calderon, Wen Li, Callen T Wallace, Ngoc Han Tran, Robert A Grassucci, Stephanie E Siegmund, Joshua Pemberton, Travis J Morgenstern, Leanna Eisenman, Jenny I Aguilar, Nili L Greenberg, Elana S Levy, Edward Yi, William G Mitchell, William J Rice, Christoph Wigge, Jyotsna Pilli, Emily W George, Despoina Aslanoglou, Maïté Courel, Robin J Freyberg, Jonathan A Javitch, Zachary P Wills, Estela Area-Gomez, Sruti Shiva, Francesca Bartolini, Allen Volchuk, Sandra A Murray, Meir Aridor, Kenneth N Fish, Peter Walter, Tamas Balla, Deborah Fass, Sharon G Wolf, Simon C Watkins, José María Carazo, Grant J Jensen, Joachim Frank, Zachary Freyberg
RESUMEN

The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.

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BRAND® 96-well microplate, U-bottom, round bottom, non-sterile