As mentioned on the Product Detail Page at the DESCRIPTION section, under 'Preparation Note' , this product is prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
T8154
Azul de tripano solution
0.4%, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
Blue cell viability dye
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Fórmula empírica (notación de Hill):
C34H24N6Na4O14S4
Número CAS:
Peso molecular:
960.81
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4360496
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Servicio técnico
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Permítanos ayudarlesterility
sterile-filtered
Quality Segment
form
liquid
storage condition
dry at room temperature
concentration
0.4%
technique(s)
cell culture | mammalian: suitable, tissue processing: suitable
application(s)
cell analysis
shipped in
ambient
storage temp.
room temp
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
General description
El azul de tripano es un colorante utilizado para distinguir entre células vivas y células muertas. Es un colorante vital que no es absorbido por las células viables sanas, pero tiñe las células con una membrana celular dañada. De esta forma, sólo pueden contarse las células muertas. Este método suele denominarse método de exclusión de colorante.
Para el mismo producto en un práctico formato liofilizado (en polvo), consúltese T6146
El azul de tripano (TB), un tinte azoico hidrófilo aniónico, atraviesa solo las membranas celulares de las células muertas, tiñendo por tanto de azul las células y los tejidos muertos. Se utiliza en microscopia cuantitativa en una técnica de tinción de exclusión de colorante para diferenciar entre células de mamíferos vivas y muertas.[1]
El azul de tripano (TB), un tinte azoico hidrófilo aniónico, atraviesa solo las membranas celulares de las células muertas, tiñendo por tanto de azul las células y los tejidos muertos. Se utiliza en microscopia cuantitativa en una técnica de tinción de exclusión de colorante para diferenciar entre células de mamíferos vivas y muertas.[1]
Application
La disolución de azul de tripano se ha utilizado :
- en el ensayo de viabilidad celular para contar células viables y muertas[2]
- en el ensayo de rescate para contar células viables[2]
- en el método de recuento por exclusión de azul tripano para determinar la curva de proliferación[3]
- como control salino y en la preparación de la disolución de lipopolisacárido (LPS) para confirmar el éxito de la infusión de LPS[4]
- en la prueba de exclusión de colorante azul tripano para determinar la viabilidad de las células epiteliales gástricas (GEC)[5]
- para contar las células de GEC utilizando un hemocitómetro[5]
Preparation Note
Se prepara en cloruro de sodio al 0,81% y en fosfato de potasio dibásico al 0,06%.
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, tissue processing: suitable | technique(s) cell culture | mammalian: suitable, tissue processing: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| concentration 0.4% | concentration - | concentration 0.4% | concentration - |
| application(s) cell analysis | application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form liquid | form powder | form liquid | form powder |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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signalword
Danger
hcodes
Hazard Classifications
Carc. 1B
Clase de almacenamiento
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| T8154-20MLSTAB | 04065272552325 |
| T8154-100ML | 04061838260109 |
| T8154-1LSTAB | 04065272481816 |
| T8154-20ML | 04061838260116 |
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is this solution dissolved in PBS?
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What is the stability of the product. How long we can use it as there is no expiry date mentioned in the COA
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
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Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
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Why is Trypan blue, Product T8154, used to determine cell viability?
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Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
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Trypan blue, Product T8154, contains a precipitate. Is it still good to use?
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The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
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Why are there red fibers in Product T8154, Trypan blue solution?
1 answer-
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
1 answer-
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
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