T2663

Trizma^® hydrochloride solution

Molecular Biology, 1 M, BioReagent

• Sinónimos: Tris hydrochloride solution
• NACRES: NA.25
• PubChem Substance ID: 24900064
• UNSPSC Code: 12161700
• MDL number: MFCD00012590

Propiedades

• grade: Molecular Biology
• Quality Segment: 200
• sterility: 0.2 μm filtered
• product line: BioReagent
• form: solution
• concentration: 1 M
• impurities: DNase, RNase, NICKase and protease, none detected
• pH: 7.35-7.45
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Descripción

General description

Trizma^® hydrochloride solution is a useful biological buffer.

Application

Trizma^® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• ¿Qué está pasando?: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type ABEK (EN14387) respirator filter