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Merck

RAB0377

Sigma-Aldrich

Visfatin EIA Kit

for serum, plasma, culture supernatant and cell lysates

Sinónimos:

Visfatin ELISA Kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

reactividad de especies

rat, human, mouse

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable
enzyme immunoassay: suitable

entrada

sample type serum
sample type culture supernatant(s)
sample type cell lysate
sample type plasma

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.778 ng/mL
standard curve range: 0.1-1000 ng/mL

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... NAMPT(10135)

Descripción general

The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.

Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.

Inmunógeno

Visfatin, synthetic peptide

Aplicación

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.

Acciones bioquímicas o fisiológicas

Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials


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Visite la Librería de documentos

Single and Concurrent Effects of Endurance and Resistance Training on Plasma Visfatin, Insulin, Glucose and Insulin Resistance of Non-Athlete Men with Obesity.
Tayebi S M, et al.
Annals of applied sport science., 4(4), 21-31 (2016)
Effects of Diet and Exercise-Induced Weight Loss in Sedentary Obese Women on Inflammatory Markers, Resistin, and Visfatin.
Khanna D, et al.
Obesity and Nutrition of J, 102 (2017)
A visfatin promoter polymorphism is associated with low?grade inflammation and type 2 diabetes.
Zhang Y-Y, et al.
Obesity (Silver Spring, Md.), 14(12), 2119-2126 (2006)
Matthaios G Pitoulias et al.
Cytokine, 91, 140-144 (2017-01-11)
Visfatin is an adipokine molecule acting as an essential coenzyme in multiple cellular redox reactions. The increased serum levels of Visfatin have been correlated with metabolic syndrome and endothelial homeostasis. In this study we investigate the possible relationship of Visfatin
Ashleigh M Philp et al.
International journal of molecular sciences, 22(13) (2021-07-03)
Obesity increases the risk of hip osteoarthritis (OA). Recent studies have shown that adipokine extracellular nicotinamide phosphoribosyltransferase (eNAMPT or visfatin) induces the production of IL-6 and matrix metalloproteases (MMPs) in chondrocytes, suggesting it may promote articular cartilage degradation. However, neither

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