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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Sinónimos:

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54

product name

Protease and Phosphatase Inhibitor Cocktail,

form

DMSO solution

Quality Level

200

packaging

pkg of 1 ml, 5 ml ×

shipped in

wet ice

storage temp.

−20°C

General description

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction
  • to lyse purified CD4+ T cells
  • to prepare protein extracts from placental tissues and cells

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Pandit M, et al.
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Endocrine-related cancer, 28(1), 1-13 (2020-10-29)
Familial primary aldosteronism (PA) is rare and mostly diagnosed in early-onset hypertension (HT). However, 'sporadic' bilateral adrenal hyperplasia (BAH) is the most frequent cause of PA and remains without genetic etiology in most cases. Our aim was to investigate new
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Mechanisms of ageing and development, 192, 111374-111374 (2020-10-06)
Fibrosis has been considered as a hallmark of dysfunctional adipose tissue (AT), however the role and mechanisms of fibrosis in the age related AT dysfunction are not yet well characterized. The aim of the study was to investigate the mechanisms
TAGLN2-regulated trophoblast migration, invasion and fusion are impaired in preeclampsia
Wang H, et al.
Frontiers in Cell and Developmental Biology, 10, 810633-810633 (2022)
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