Yes, a suitable chromogenic substrate for the enzyme associated with product number P6556 is the S7388, N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide.
P6556
Proteinasa K from Tritirachium album
lyophilized powder, ≥30 units/mg protein
Sinónimos:
Endopeptidasa K
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10 MG
$49.100
5 MG
$55.500
25 MG
$78.700
100 MG
$129.000
500 MG
$551.000
1 G
$1.240.000
Seleccione un Tamaño
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10 MG
$49.100
5 MG
$55.500
25 MG
$78.700
100 MG
$129.000
500 MG
$551.000
1 G
$1.240.000
About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Productos recomendados
form
lyophilized powder
Quality Level
specific activity
≥30 units/mg protein
mol wt
28.93 kDa
technique(s)
DNA extraction: suitable
solubility
H2O: soluble 1 mg/mL, clear, colorless
foreign activity
Dnase ≤30 Kunitz units/mg solid
RNase ≤0.003 Kunitz units/mg solid
shipped in
wet ice
storage temp.
−20°C
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Application
Útil para la inactivación proteolítica de nucleasas durante el aislamiento de ADN y ARN.
Útil para la inactivación proteolítica de nucleasas durante el aislamiento de ADN y ARN.
Elimina las endotoxinas que se unen a las proteínas catiónicas como la lisozima y la ribonucleasa A.
Se ha comunicado que es útil para el aislamiento de mitocondrias hepáticas, de levadura y de frijol mung
Determinación de la localización de las enzimas en las membranas
Tratamiento de cortes de tejido incluidos en parafina para exponer los sitios de unión de antígenos para el marcado con anticuerpos.
Digestión de proteínas de muestras de tejido cerebral para investigación de los priones en las encefalopatías espongiformes transmisibles (EET).
Elimina las endotoxinas que se unen a las proteínas catiónicas como la lisozima y la ribonucleasa A.
Se ha comunicado que es útil para el aislamiento de mitocondrias hepáticas, de levadura y de frijol mung
Determinación de la localización de las enzimas en las membranas
Tratamiento de cortes de tejido incluidos en parafina para exponer los sitios de unión de antígenos para el marcado con anticuerpos.
Digestión de proteínas de muestras de tejido cerebral para investigación de los priones en las encefalopatías espongiformes transmisibles (EET).
Product P6556 is provided as a lyophilized powder. Product P6556 has been used to break down human lens protein[1]. Protease footprinting by Proteinase K digestion can reveal protein-protein surface interactions.[2] The enzyme from Sigma has been used in the pre-hybridization step of chicken embryos.[3] It has also been used for the enrichment of PrPSc, a prion protein that is present in sheep, hamster and mouse scrapie samples.[4]
Proteinase K is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
It is used for the removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.
It is useful for the isolation of hepatic, yeast, and mung bean mitochondria
and is used to determine enzyme localization on membranes
It is used for the treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling and
for digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Product P6556 is provided as a lyophilized powder. Product P6556 has been used to break down human lens protein[1].
It is used for the removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.
It is useful for the isolation of hepatic, yeast, and mung bean mitochondria
and is used to determine enzyme localization on membranes
It is used for the treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling and
for digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Product P6556 is provided as a lyophilized powder. Product P6556 has been used to break down human lens protein[1].
Biochem/physiol Actions
La proteinasa K es una serín proteasa estable y muy reactiva. La evidencia procedente de estudios de la estructura cristalina y molecular indica que la enzima pertenece a la familia subtilisina con una tríada catalítica en el sitio activo (ASP39-HIS69-ser224). Es estable en una amplia variedad de entornos: pH, sales amortiguadoras, detergentes (SDS) y temperatura. En presencia de SDS al 0,1 - 0,5 %, la proteinasa K conserva su actividad y digerirá una variedad de proteínas y nucleasas en preparaciones de ADN sin comprometer la integridad del ADN aislado.
Proteinase K has a broad specificity and degrades many proteins even in the native state. It mainly cleaves the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked α-amino groups.[4][5][6] The optimum pH is between 7.5-9.0 and the isoelectric point is 8.9[4] Ca2+ (1-5 mM) is required for activation. Proteinase K is inhibited by diisopropyl fluorophosphate (DFIP), and phenylmethanesulfonyl fluoride (PMSF).[5]
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
also commonly purchased with this product
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Certificados de análisis (COA)
Lot/Batch Number
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Los clientes también vieron
Nathaniel Denkers et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 229(3), 651-657 (2004-03-03)
Multi-color whole-mount in situ hybridization is a powerful technique for comparing the spatial expression patterns of two or more genes in developing embryos. We have developed an amplified triple-label whole-mount fluorescence in situ hybridization (FISH) protocol that permits detection of
M M Kristjánsson et al.
European journal of biochemistry, 260(3), 752-760 (1999-04-02)
An extracellular serine proteinase purified from cultures of a psychrotrophic Vibrio species (strain PA-44) belongs to the proteinase K family of the superfamily of subtilisin-like proteinases. The enzyme is secreted as a 47-kDa protein, but under mild heat treatment (30
H Hilz et al.
European journal of biochemistry, 56(1), 103-108 (1975-08-01)
Hydrolysis of serum albumin by proteinase K was strongly (greater than 7-fold) stimulated by urea and dodecylsulfate in a dose-dependent manner. With an oligopeptide as substrate, however, proteinase K was inactivated by dodecylsulfate. This indicates that the apparent activation of
Enzymes of Molecular Biology
M.M. Burrell
Methods in Molecular Biology, 16, 307-307 (1993)
Janus S Jakobsen et al.
Science advances, 5(7), eaaw4304-eaaw4304 (2019-07-17)
The key myeloid transcription factor (TF), CEBPA, is frequently mutated in acute myeloid leukemia (AML), but the direct molecular effects of this leukemic driver mutation remain elusive. To investigate CEBPA mutant AML, we performed microscale, in vivo chromatin immunoprecipitation sequencing
Artículos
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.
Protocolos
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
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Is there a chromogenic substrate available for product number P6556?
1 answer-
Helpful?
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How can I activate the proteinase K? I need it for a protease assay
1 answer-
Product P6556, Proteinase K, is activated by 0.5 % SDS or 6 M urea.
Helpful?
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Is your "Proteinase K from Tritirachium album" actually isolated from Tritirachium album cultures, or the gene cloned, over-expressed in some bacterium and the Proteinase K isolated from the bacterial cell lysate?
1 answer-
This product is isolated from Tritirachium album culture.
Please see the sample Certificate of Origin below:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COO/Q12/P6556-1G0000137404.pdfHelpful?
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