P5936

Pectolyase from Aspergillus japonicus

suitable for plant cell culture, lyophilized powder

• Sinónimos: PEL1
• UNSPSC Code: 10171502
• NACRES: NA.72
• Número CE: 3.2.1.15 (BRENDA, IUBMB)
• MDL number: MFCD00131813
• Specific activity: ≥0.3 unit/mg solid

Propiedades

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥0.3 unit/mg solid
• greener alternative product characteristics: Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• greener alternative category: Enabling,
• storage temp.: 2-8°C

Descripción

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)