P5538

Phosphoglucose Isomerase from Bacillus stearothermophilus

lyophilized powder, 300-1,000 units/mg protein

• Sinónimos: D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
• Número CAS: 9001-41-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-603-1
• MDL number: MFCD00131887
• Número CE: 5.3.1.9 (BRENDA, IUBMB)
• Specific activity: 300-1,000 units/mg protein

Propiedades

• form: lyophilized powder
• Quality Segment: 200
• specific activity: 300-1,000 units/mg protein
• mol wt: 189 kDa
• composition: Protein, ≥60% biuret
• storage temp.: −20°C

Descripción

General description

The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.

Application

Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus.

The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.

Biochem/physiol Actions

Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Physical form

lyophilized powder containing Tris buffer

Other Notes

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)