P5381

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

• Sinónimos: D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
• Número de CAS: 9001-41-6
• Comisión internacional de enzimas: 5.3.1.9 (BRENDA, IUBMB)
• Número CE: 232-603-1
• Número MDL: MFCD00131887
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bakers yeast
• Nivel de calidad: 200
• tpo: Type III
• Formulario: ammonium sulfate suspension
• actividad específica: ≥400 units/mg protein (biuret)
• mol peso: tetramer 119,500 Da±600
• concentración: ≥3 mg/mL protein Biuret
• técnicas: activity assay: suitable
• color: white
• Nº de acceso Protein ID: NP_009755.1
• Nº de acceso UniProt: P12709
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: yeast ... PGI1(852495)

Descripción

Descripción general

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Aplicación

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:

• to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
• as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
• to convert fructose to glucose while evaluating fructose content in kudzu roots.

Acciones bioquímicas o fisiológicas

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Definición de unidad

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

Forma física

Crystalline suspension in 2.6 M (NH₄)₂SO₄ containing 0.1 mM magnesium acetate, pH 7.0

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves