This product has not been specifically tested for freeze/thaw tolerance in solution. However, product 540025, which is Pluronic F-127 10% solution in water, is suitable for freezing. A 10% solution of Pluronic F-127 in phosphate-buffered saline is expected to be stable when stored in aliquots at –20°C. Avoid repeated freeze/thaw cycles.
P2443
Pluronic® F-127
powder, BioReagent, suitable for cell culture
Sinónimos:
Non-ionic copolymer surfactant
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
250 g | Disponible para enviar HOYdesdePUDAHUEL | $105.000 |
1 kg | Comprobar disponibilidad del carrito | $241.000 |
Acerca de este artículo
Fórmula lineal:
(C3H6O·C2H4O)x
Número CAS:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Servicio técnico
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Permítanos ayudarleQuality Segment
description
Non-ionic, contains 100ppm BHT
product line
BioReagent
form
powder
mol wt
~12600 g/mol
technique(s)
cell culture | mammalian: suitable
CMC
950-1000 ppm (~25°C)
transition temp
cloud point >100 °C
HLB
18 - 23
SMILES string
O2C(C2)C.O1CC1
InChI
1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2
InChI key
RVGRUAULSDPKGF-UHFFFAOYSA-N
General description
Pluronicpluronic® F-127, también conocido como poloxámero 407, es un copolímero no tóxico[1] y no iónico. Contiene óxido de polietileno-óxido de polipropileno dispuesto en una estructura tribloque para formar PEO-PPO-PEO simétrico. Las propiedades tensioactivas de este polímero, derivadas de su naturaleza anfifílica, le permiten interactuar con superficies hidrófobas y membranas biológicas.
Application
Pluronic® F-127 se ha utilizado:
- como un sistema de administración de un péptido al sitio de la herida para estudiar el efecto del péptido en la cicatrización de las heridas en ratones.
- como revestimiento superficial para sellos de PDMS (polidimetilsiloxano) para volverlos repelentes a hidrogel.[2]
- en la preparación de disolución indicadora de calcio de reserva para medir las actividades neuronales.
- como componente de la disolución salina tamponada con fosfato (PBS), para reducir la adhesión celular y proteica inespecífica a un dispositivo microfluídico basado en PDMS.[3]
- Este copolímero tensioactivo no iónico es adecuado para utilizar como antiespumante en aplicaciones de cultivo de células de insectos.
Biochem/physiol Actions
Pluronic® F-127 es un gel termorreversible que encuentra su aplicación en diferentes industrias debido a su gran termosensibilidad, biodegradabilidad y biocompatibilidad.[1] Estas propiedades hacen que el polímero sea adecuado para utilizar como transportador versátil de medicamentos. Pluronic® F-127 mejora la eficiencia terapéutica de los medicamentos al aumentar su biodisponibilidad y su termoestabilidad. Además, puede promover la unión celular y la formación de colágeno, lo que facilita la angiogénesis y la regeneración tisular. Debido a su eficacia, Pluronic F-127 se ha utilizado en la encapsulación de células madre mesenquimatosas (MSCs) para ayudar en la regeneración de tejidos con bajos niveles de vascularidad, como los tejidos epiteliales, los tejidos adiposos, los cartílagos, los tendones y los tejidos óseos.[1]
Legal Information
Pluronic is a registered trademark of BASF
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) cell culture | mammalian: suitable |
| form powder | form - | form solid | form solid, waxy solid |
| description Non-ionic, contains 100ppm BHT | description - | description non-ionic | description - |
| mol wt ~12600 g/mol | mol wt - | mol wt - | mol wt - |
| CMC 950-1000 ppm (~25°C) | CMC - | CMC - | CMC - |
| HLB 18 - 23 | HLB - | HLB - | HLB - |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can I aliquot and freeze pluronic 10% (in PBS) at -20°C?
1 answer-
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Pluroni F-127 어떤 solution에 녹여서 stock 만들어야 되나요? D.W 인가요? DMSO 인가요? PBS 인가요?
1 answer-
This surfactant is soluble in water at >10% at room temperature. The product solubility is tested at 50 mg/mL in water. Similar rates are expected in PBS. The solubility in DMSO has not been investigated.
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Is Pluronic® F-127 soluble in water and/or KOH solutions (up to 10M)?
1 answer-
This product is tested for solubility at 50 mg/mL in water at temperatures less than 10°C. Maximum solubility has not been tested.
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What is the hydroxyl number of F127?
1 answer-
Based on an approximate molecular weight of 12,600, this block co-polymer with two hydroxyl groups has a theoretical hydroxyl value of 8.9 mg potassium hydroxide (KOH) per gram of polymer. Higher experimental results for the hydroxyl value would indicate some branching.
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Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?
1 answer-
There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:
Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756
Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162
Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853
These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.
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I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?
1 answer-
The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:
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can I know what is the chain length of each PEO and PPO of this F-127?
1 answer-
Pluronic F 127 corresponds to the structure of Kolliphor P 407.
This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
A = 101
B = 56Helpful?
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What is the VOC content of this product?
1 answer-
Volatile Organic Compound (VOC) content is not measured for this product.
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Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?
1 answer-
The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.
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What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?
1 answer-
The solution can be sterilized with a 0.22 uM sterile filter.
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