P1867

Plasmin from human plasma

lyophilized powder, ≥2.0 units/mg protein

• Sinónimos: Fibrinolysin
• Número CAS: 9001-90-5
• UNSPSC Code: 12352204
• eCl@ss: 42030239
• EC Number: 232-640-3
• NACRES: NA.54
• MDL number: MFCD00131921
• Número CE: 3.4.21.7 (BRENDA, IUBMB)
• Specific activity: ≥2.0 units/mg protein

Propiedades

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥2.0 units/mg protein
• impurities: HIV, Hepatitis B and Hepatitis C, tested negative
• UniProt accession no.: P00747
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: human ... PLG(5340)

Descripción

General description

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes.

Application

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin.

Biochem/physiol Actions

It is inhibited by α 2-antiplasmin and its interaction with fibrin is blocked. It digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells.

Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.
pH Optimum: 8.5
pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity
Temperature Optimum: 37 °C with rapid inactivation at 56 °C

Packaging

Package size based on protein content

Physical form

20 mM sodium phopshate, pH 7.4, with 10 mg/ml D-Mannitol and 10 mg/ml NaCl

Other Notes

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable