N3786

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

• Sinónimos: Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase
• Número CAS: 9001-67-6
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-624-6
• MDL number: MFCD00131711
• Número CE: 3.2.1.18 (BRENDA, IUBMB)
• Specific activity: ≥25 U/vial

Propiedades

• form: lyophilized powder
• Quality Segment: 200
• quality: Proteomics Grade
• specific activity: ≥25 U/vial
• suitability: suitable for MALDI-TOF MS
• storage temp.: 2-8°C

Descripción

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Physical form

Lyophilized powder

Analysis Note

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Other Notes

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Los componentes del kit también están disponibles por separado

• N3536

  Neuraminidase 5X Reaction Buffer 1.5 mL

  SDS

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable