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Merck

MTOX1300

Canine MDCKII Wild Type Cells

canine

Sinónimos:

MDCK II, MDCK-2, MDCKII-WT

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UNSPSC Code:
12352207
NACRES:
NA.81
Biological source:
canine
Servicio técnico
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Nombre del producto

Canine MDCKII Wild Type Cells,

biological source

canine

Quality Segment

form

liquid

storage temp.

−196°C

General description

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435).

Application

Canine MDCKII Wild Type Cells can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. See technical bulletin for detailed protocols.

Features and Benefits

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron.

Preparation Note

Upon receiving a shipment of frozen canine MDCKII wild type cells it is important the end user gives the shipment attention without delay. To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70 °C. Storage at –70 °C will result in loss of viability.

Analysis Note

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

Exhibit 1: ADME/Tox cell lines licenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.


Clase de almacenamiento

10 - Combustible liquids

¿Qué está pasando?

WGK 3

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable



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