If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
MAK121
Neuraminidase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Sinónimos:
Sialidase Activity Assay Kit
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Comprobar disponibilidad del carrito | $734.000 |
Acerca de este artículo
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
pharmaceutical
detection method
colorimetric, fluorometric
shipped in
dry ice
storage temp.
−20°C
General description
Application
- 5-Nitrobenzo[c][1, 2, 5]selenadiazole as therapeutic agents in the regulation of oxidative stress and inflammation induced by influenza A(H1N1)pdm09 in vitro and in vivo.: This research utilized the Neuraminidase Activity Assay Kit to explore therapeutic agents that regulate oxidative stress and inflammation, showcasing its crucial role in antiviral research and potential pharmacological applications (Guo et al., 2023).
- Potency of bacterial sialidase Clostridium perfringens as antiviral of Newcastle disease infections using embryonated chicken egg in ovo model.: Highlights the kit′s use to measure and observe sialidase enzyme activity to assess the antiviral potential of bacterial enzymes against poultry diseases, contributing to the development of novel biocontrol methods (Kurnia et al., 2022).
- Molecular Docking Studies and Biological Evaluation of Berberine-Benzothiazole Derivatives as an Anti-Influenza Agent via Blocking of Neuraminidase.: Utilizes neuraminidase assay kit to evaluate the efficacy of synthesized compound′s neuraminidase activity, offering a path towards the design of new antiviral drugs with enhanced specificity and potency (Kumar et al., 2021).
Biochem/physiol Actions
Features and Benefits
1 of 1
Este artículo | |||
|---|---|---|---|
| usage sufficient for 100 colorimetric or fluorometric tests | usage sufficient for 100 colorimetric or fluorometric tests | usage - | usage sufficient for 100 tests (Ultraviolet) |
| detection method colorimetric, fluorometric | detection method colorimetric, fluorometric | detection method - | detection method colorimetric |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in dry ice | shipped in - | shipped in - | shipped in dry ice |
| application(s) pharmaceutical | application(s) cosmetics | application(s) diagnostic assay manufacturing | application(s) - |
signalword
Danger
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 2
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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What is the pH of the assay buffer?
1 answer-
The assay buffer is a pH of 7.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is it possible to use the Neuraminidase Activity Assay Kit (MAK121-1KT) for testing bacterial cultures, given that references indicate the use of plasma, serum, and purified enzymes as samples?
1 answer-
The kit has been verified for use with bacterial cultures. Although there isn't a tailored sample preparation protocol for bacterial cultures, the following general cell lysate preparation method is suggested:
To prepare cell samples, first, suspend approximately two million harvested cells (2 × 10^6) in 400 μL of PBS on ice. Cell lysis can be accomplished by either using a Dounce homogenizer for 10-20 passes while on ice or through sonication in an ice-water bath. The effectiveness of lysis should be verified under a microscope. After lysis, centrifuge the homogenate at 14,000 g for 10 minutes and then transfer the clear supernatant to a clean tube. It is recommended to run a pilot assay with different sample dilutions to identify a dilution that falls within the linear range of the standard curve for accurate assays. If immediate analysis is not possible, most samples can be preserved at -80°C.Helpful?
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What type of sialic acid linkage is present in this kit? Specifically, is it an α2,3- or α2,6-linkage?
1 answer-
The substrate utilized is fetuin, which is a heavily sialylated protein derived from fetal bovine serum. The specific type of sialic acid linkage present in this substrate has not been characterized. However, fetuin typically exhibits two types of linkages: α2,3- and α2,6-linkages. It is more common for α2,3-linkages to be present in fetuin substrates, which suggests that experiments targeting this linkage type are likely to be successful. Nonetheless, it is not possible to provide a definitive statement on the exact type of linkage present.
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Hallo, welches MQ Level hat das Material MAK121?
1 answer-
These kits is considered an out of scope in terms of the MQ Classification system, but are handled and supported similarly to the MQ100 level products.
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