M9400
β-Mannosidase from Helix pomatia
5-30 units/mL, ammonium sulfate suspension, crude extract
Sinónimos:
β-D-Mannoside mannohydrolase
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32
Productos recomendados
Formulario
ammonium sulfate suspension
Nivel de calidad
calidad
crude extract
mol peso
94 kDa
concentración
5-30 units/mL
actividad extraña
β-N-acetylglucosaminidase and α-mannosidase <1.0%
temp. de almacenamiento
2-8°C
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Descripción general
β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.
Aplicación
β-Mannosidase from Helix pomatia has been used:
- in the digestion of glycans for enzymatic analysis
- in the treatment of labeled monogalactosyl Manno-oligosaccharides
- in non-mammalian glycosidase inhibition assays
Acciones bioquímicas o fisiológicas
β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.
Definición de unidad
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
Forma física
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
Inhibidor
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Roneel Prakash et al.
Journal of plant physiology, 169(12), 1125-1133 (2012-06-05)
Cell walls of tomato fruit contain hemicellulosic mannans that may fulfill a structural role. Two populations were purified from cell walls of red ripe tomato tissue and named galactoglucomannan-glucuronoxylan I and II (GGM-GX I and II), respectively. Both polysaccharides not
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Applied biochemistry and biotechnology, 167(1), 73-80 (2012-04-25)
A novel biosensor strain (Escherichia coli ALM403) that responded to N-acyl homoserine lactone (AHL) was constructed using a luxR-Plux cassette as a regulatory sequence and β-mannanase as a reporter gene. Dinitrosalicylic acid method was used to detect the response of
A A Klyosov et al.
Carbohydrate research, 352, 65-69 (2012-03-23)
Statistical modeling was applied for describing structural features of β-(1→4)-D-galactomannans. According to the model suggested theoretical ratios of limiting degrees of locust bean, tara gum and guar gum galactomannan conversions by two β-(1→4)-mannanases of different origin (Myceliophthora thermophila and Trichoderma
P Wongputtisin et al.
Journal of applied microbiology, 113(4), 798-806 (2012-07-14)
To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated
Umme Afsari Zahura et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 162(1-3), 24-33 (2012-03-01)
A β-D-mannosidase (EC 3.2.1.25) with a molecular mass of approximately 100 kDa was purified from the digestive fluid of a marine gastropod Aplysia kurodai by ammonium sulfate fractionation followed by column chromatographies on TOYOPEARL Butyl-650 M, TOYOPEARL DEAE-650 M, and
Artículos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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