Saltar al contenido
Merck

M6018

Sigma-Aldrich

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

Sinónimos:

4-Methylumbelliferyl beta-D-cellobioside

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Fórmula empírica (notación de Hill):
C22H28O13
Número de CAS:
Peso molecular:
500.45
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

4-Methylumbelliferyl β-D-cellobioside, glucanase substrate

Quality Level

assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
  • to assay cellulase from A. niger
  • to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
  • to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Methylxanthine Drugs Are Chitinase Inhibitors: Investigation of Inhibition and Binding Modes
Rao FV, et al.
Chemistry & Biology, 12(9), 973-980 (2005)
Reconciling opposing soil processes in row-crop agroecosystems via soil functional zone management
Williams A, et al.
Agriculture, Ecosystems & Environment, 236, 99-107 (2017)
G P Xue et al.
Journal of general microbiology, 138(11), 2397-2403 (1992-11-01)
A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to
M Pedraza-Reyes et al.
Archives of microbiology, 168(4), 321-327 (1997-09-23)
The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched preparations of
K H Jung et al.
Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico