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M3895

Mucin from bovine submaxillary glands

Type I-S

Sinónimos:

MUC

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Número CAS:
84195-52-8
UNSPSC Code:
12352202
EC Number:
282-357-4
NACRES:
NA.61
MDL number:
MFCD00131629
Form:
powder
Biological source:
bovine submaxillary glands
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biological source

bovine submaxillary glands

Quality Segment

200

type

Type I-S

form

powder

composition

Bound sialic acids, 9-24%

technique(s)

electrophoresis: suitable

UniProt accession no.

P98091

storage temp.

−20°C

Gene Information

cow ... BSM(286841)

General description

Neuraminidase substrate.
Mucin from bovine submaxillary glands is encoded by two bovine submaxillary BSM genes. It is present in the saliva and interacts with air and food. Mucin comprises three major domains namely N- and C terminal and the glycosylated proline, threonine and serine (PTS domain) central domain. The sialic acid contributes to 30% of the molecular weight of mucin. The central domain has many tandem repeats.

Application

Mucin from bovine submaxillary glands has been used:
  • in the quantification of mucin
  • as a component of artificial tear solution
  • as a substrate for recombinant sialidase Gardnerella vaginalis
  • to investigate a galactose-specific lectin from the red marine alga Ptilota filicina
  • to study the characterization of a unique mucin-like glycoprotein secreted by a human endometrial adenocarcinoma cell line (Ishikawa)

Biochem/physiol Actions

Albuminoid substance in mucus which gives it its ropy consistency
Bovine submaxillary mucin (BSM) is thermally stable up to 85°C. The terminal domain regions are key for the adsorption functionality of mucin.

Other Notes

Similar to M 4503, but produced by Sigma.

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Este artículo
499643M1778A7517
Mucin from bovine submaxillary glands Type I-S

Sigma-Aldrich

M3895

Mucin from bovine submaxillary glands

Mucin, Bovine Submaxillary Gland Native mucin from bovine submaxillary gland. It is a rich source of O-linked oligosaccharide chains and it is suitable as a substrate for neuraminidase.

Sigma-Aldrich

499643

Mucin, Bovine Submaxillary Gland

Mucina from porcine stomach Type III, bound sialic acid 0.5-1.5 %, partially purified powder

Sigma-Aldrich

M1778

Mucina from porcine stomach

Seroalbúmina bovina For use as a marker in SDS-PAGE

Sigma-Aldrich

A7517

Seroalbúmina bovina

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

electrophoresis: suitable

biological source

bovine submaxillary glands

biological source

-

biological source

Porcine stomach

biological source

bovine

form

powder

form

lyophilized

form

partially purified powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

UniProt accession no.

P98091

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P02769

Gene Information

cow ... BSM(286841)

Gene Information

-

Gene Information

-

Gene Information

bovine ... ALB(280717)

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. Is this protein purified using His-tag or some other tag?

    1 answer

    1. The detailed protein purification process for this product is considered proprietary. This material is a native-source (non-recombinant) animal product with no affinity tag. The purification process is a modification of a CTAB-precipitation method of Tettamanti & Pigman (Arch. Biochem. Biophys. 124:41–50, 1968) and is then dialyzed against water (no buffer salts). The final pH is approximately 5–6. There are no buffer salts present in the final product and the material is not purified under native conditions.

      Pubmed link : https://pubmed.ncbi.nlm.nih.gov/5661614/

      Helpful?

  2. What is the recommended method to dissolve Mucin from bovine submaxillary glands, item M3895?

    1 answer

    1. Mucin from bovine submaxillary glands, item M3895, can be dissolved/reconstituted in H2O or dilute aqueous alkaline buffers.

      Helpful?

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