L5505

Lectin from Ulex europaeus (gorse, furze)

lyophilized powder

• Sinónimos: Ulex europaeus agglutinin, UEA-I
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder

Propiedades

• form: lyophilized powder
• Quality Segment: 200
• potency: <8 μg/mL agglutination activity
• composition: Protein, ≥80% biuret
• impurities: salt, essentially free
• storage temp.: 2-8°C

Descripción

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Preparation Note

Purified by affinity chromatography using the method of Pereira.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Other Notes

Conjugates are prepared from affinity purified lectin.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)