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K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

Código UNSPSC:
12161702
NACRES:
NA.75

Nivel de calidad

500

Formulario

powder

técnicas

cell culture | mammalian: suitable

pH

6.1-6.7

solubilidad

water: 9.5 g/L, clear

temp. de almacenamiento

2-8°C

Descripción general

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Aplicación

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Cantidad

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitución

Supplement with 1.26 g/L sodium bicarbonate.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000347442
0000347442
SLCP6729
SLCG7890
SLCB4396

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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