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H7787

HIS-Select® Spin Columns

Sinónimos:

His-Tag Spin Columns

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A ustedes/SKUDisponibilidadPrecio
10 ea
Comprobar disponibilidad del carrito
$195.000
50 ea
Comprobar disponibilidad del carrito
$653.000

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NACRES:
NA.56
UNSPSC Code:
41106500
Pore size:
100 nm pore size
Packaging:
pkg of 10 ea, pkg of 50 ea

$195.000


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shelf life

2 yr at 2‑8 °C

Quality Segment

packaging

pkg of 10 ea, pkg of 50 ea

pore size

100 nm pore size

capacity

>0.5 mg/column

storage temp.

2-8°C

General description

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic cell lysis reagents

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
SC1000S4574Z629103
pore size

100 nm pore size

pore size

-

pore size

-

pore size

3-5 μm

packaging

pkg of 10 ea, pkg of 50 ea

packaging

pkg of 1 kit

packaging

-

packaging

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

shelf life

2 yr at 2‑8 °C

shelf life

-

shelf life

-

shelf life

-

storage temp.

2-8°C

storage temp.

20-25°C

storage temp.

room temp

storage temp.

-

capacity

>0.5 mg/column

capacity

-

capacity

-

capacity

4 mg binding capacity


Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. Can HIS-Select® Spin Column, Product H7787, be re-used?

    1 answer
    1. We do not recommend re-using the columns. Each column is intended for one time use.

      Helpful?

  2. What is the binding capacity of the HIS-Select® Spin Column, Product H7787?

    1 answer
    1. The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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