If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
F6428
Reactivo glicerol libre
used for quantitative enzymatic determination of glycerol
Seleccione un Tamaño
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
40 mL | Comprobar disponibilidad del carrito | $179.000 |
Acerca de este artículo
Quality Segment
form
solid
usage
40 mL sufficient for 50 reactions
color
white to off-white
functional group
hydroxyl
relevant disease(s)
obesity
storage temp.
2-8°C
SMILES string
C(C(CO)O)O
InChI
1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
InChI key
PEDCQBHIVMGVHV-UHFFFAOYSA-N
General description
El kit de determinación de triglicéridos séricos (número de referencia TR0100) ofrece una solución integral para evaluar el glicerol, los triglicéridos verdaderos o los triglicéridos totales en muestras de suero o plasma. Utilizando la hidrólisis enzimática con lipasa, los triglicéridos se convierten en glicerol y ácidos grasos libres, permitiendo una cuantificación precisa mediante reacciones enzimáticas acopladas por F6428.
A diferencia de muchos reactivos de triglicéridos comercializados, este método distingue entre el glicerol endógeno y el glicerol derivado de la hidrólisis de glicéridos con lipasa. Con esta avanzada tecnología, los investigadores pueden analizar con confianza los niveles de glicerol con una precisión y fiabilidad excepcionales.
Application
Biochem/physiol Actions
Features and Benefits
- Proceso simplificado: optimice su secuencia de trabajo con un solo reactivo, ofreciendo un tiempo de reacción rápido de 5 minutos.
- Diseño fácil de usar: elimina la necesidad de pesar o mezclar varios reactivos, garantizando la facilidad de uso y la eficiencia.
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| functional group hydroxyl | functional group - | functional group - | functional group - |
| form solid | form - | form - | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color white to off-white | color - | color - | color - |
| relevant disease(s) obesity | relevant disease(s) - | relevant disease(s) - | relevant disease(s) - |
| usage 40 mL sufficient for 50 reactions | usage - | usage kit sufficient for 1000 reactions | usage - |
signalword
Danger
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
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How can I determine the shelf life / expiration / retest date of this product?
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I would like to measure the concentration of free glycerol in the culture supernatant of adipocytes using this reagent with a plate reader instead of a cuvette. What are recommended sample and reagent volumes for a plate reader? Have you ever tested?
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This product has not been tested for use with a well plate format. The product instructions call for a total volume of 830 µL, where 10 µL each of water, glycerol standard, and sample are added to cuvettes containing 800 µL of Free Glycerol Reagent. Most 96-well plates have a working volume of 100-300 µL, so the volume of each component would need to be scaled accordingly. Adding 3 µL each of water, glycerol standard, and sample to wells containing 240 µL of Free Glycerol Reagent would result in the same concentrations with a total volume of 249 µL. Regardless, the functionality of a plate reader in this application cannot be guaranteed and would need to be assessed independently.
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I have an old version of this product (6428) - just one bottle not a kit. I need to reconstitute this but can't find instructions. Can you let me know what to reconstitute in and how much? Thank you!
1 answer-
There are no specific instructions for 6428. F6428 is one bottle of reagent as well. The user guide of F6428 may serve as a reference. A typical reconstitution procedure of F6428 is: Reconstitute the Free Glycerol Reagent with 40 ml of water. After addition of water, stopper the vials, and immediately mix several times by inversion. DO NOT SHAKE. Protect the reagent from light by storing in amber bottles. Please check page 2 of the user guide here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/352/968/f6428bul.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Could you kindly send me a package insert and a manual application for determination on automated clinical systems? Many thanks.
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The Free Glycerol Reagent is for R&D use only, not for in vitro diagnostic use, drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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