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Merck

F4648

Sigma-Aldrich

Fast Red TR/Naphthol AS-MX

SIGMAFAST, fluorogenic, tablet

Sinónimos:

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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5 SETS
$40.600
50 SETS
$147.000

$40.600


Fecha estimada de envío01 de abril de 2025



Seleccione un Tamaño

Cambiar Vistas
5 SETS
$40.600
50 SETS
$147.000

About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.83

$40.600


Fecha estimada de envío01 de abril de 2025


Nombre del producto

SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL

Quality Level

form

tablet

solubility

water: 1 tablet/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

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Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
  • in alkaline phosphatase staining in organ sections[1]
  • for staining chondrogenic spheroids in immunohistochemistry studies[2]
  • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections[3]

Reconstitution

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Artículos

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

  1. Could product F4648 be used with a 543 laser system?

    1 answer
    1. When considering your question, the initial response was that Fast Red TR is typically used for light microscopy. I consulted Conn's Biological Stains, 10th Edition from the Biological Stain Commission and found a reference for its use in fluorescence microscopy in an article by Lizard, G et al (1997). The article is titled "Detection of human papillomavirus DNA in genital lesion by enzymatic in situ hybridization with fast red and laser confocal microscopy" and was published in the Histochemical Journal (29: 544-554).

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