F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

• Sinónimos: Fibronectin
• Número CE: 289-149-2
• Número MDL: MFCD00131062
• Código UNSPSC: 12352202
• NACRES: NA.75

Propiedades

• Nombre del producto: Fibronectina, lyophilized powder, suitable for cell culture
• origen biológico: human foreskin fibroblasts
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Formulario: lyophilized powder
• mol peso: ~550 kDa
• envase: pkg of 0.5 mg
• técnicas: cell culture | mammalian: suitable
• cobertura de la superficie: 1‑5 μg/cm²
• impurezas: Human Source, none detected (HCV); Human Source, none detected (HIV); Human Source, none detected (HTLV); Human Source, none detected (HbSAg); Microbial Contamination, passes test
• solubilidad: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• Nº de acceso NCBI: NM_212482.4
• Nº de acceso UniProt: P02751
• Especificidad de unión: Peptide Source: Collagen
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... FN1(2335)

Descripción

Descripción general

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Aplicación

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Acciones bioquímicas o fisiológicas

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Características y beneficios

• Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
• It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
• The starting material is tested and found negative for HIV and HBsAg.
• The product is soluble in sterile water, yielding a clear, colorless solution.
• It can be stored at -20°C for at least three years.

Precaución

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Forma física

Lyophilized in CAPS buffered saline

Nota de preparación

dialyzed against chloroform

Reconstitución

Store reconstituted solution in working aliquots at -20°C or lower.

Nota de análisis

Protein homogeneity is evaluated by immunoelectrophoresis.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)